C2259-04A
CD8 (Leu2, MAL, p32, T-cell surface glycoprotein CD8 alpha chain, T-lymphocyte differentiation antigen T8/Leu-2) (Preservative Free)
Size
£479.00
- SKU:
- C2259-04A
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human thymocytes/Sezary T cells
- Clone:
- UCHT4
- Format:
- Supplied as a liquid.. No preservative added.
- Extra Details:
- Human CD8 is a heterodimeric protein consisting of an alpha and beta chain, each about 33kD. CD8 is expressed on most thymocytes and on about one third of peripheral blood T cells. CD8 is a co-receptor involved in antigen recognition and binds to the alpha 2 and alpha 3 domain of MHC Class I molecules (3, 4). Applications: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: ELISA: Capture Flow Cytometry: 5x10e5 cultured human HPB-MLT cells per tube were washed and pre-incubated with 20ul human IgG at 250ug/ml (to block non specific binding) after which they were incubated 45min on ice with 80ml of anti-CD8 antibody at a concentration of 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.67 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice