C2258-94K
CD8a (Ly2, T cell Co-receptor, Leu2, T8)
Size
£422.00
- SKU:
- C2258-94K
- Purity:
- Supernatant.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Synthetic peptide corresponding to the C-terminal region of the human CD8 alpha chain, coupled to a N-terminal cysteine, with the sequence C-KSDGKPSLSARYV, coupled to BSA and KLH.
- Clone:
- 4B11
- Format:
- Supplied as a liquid in 0.09% sodium azide.
- Extra Details:
- CD8 cell surface antigen is a ~32kD glycoprotein expressed by the cytotoxic/suppressor subset of T-cells and weakly by NK cells. This anitbody was raised based on an earlier successful strategy used to generate rabbit polyclonal antibodies against human CD8 employing the same immunizing peptide (Mason et al. 1992). Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (frozen): 1:50 Immunohistochemistry (paraffin): 1:50, requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 8.0 is recommended for this purpose. Western Blot: 1:25 Optimal working dilutions to be determined by researcher. Positive Control Tissue: Human Tonsil. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice