C2258-94G

CD8 (p32, LEU2)

Size

£479.00

SKU:: C2258-94G
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human blood lymphocytes
Clone:: LT8
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Extra Details:: The CD8 T cell coreceptor (monomer ~32-34kD) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling. Applications: Suitable for use in Flow Cytometry and Immunoprecipitation. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1-4ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.