C2258-90M-BIOTIN
CD8 alpha (Leu2 T lymphocyte antigen, MAL, OKT8 T Cell antigen, p32, T cell antigen Leu2, T cell surface glycoprotein CD8 alpha) (Biotin)
Size
£856.00
- SKU:
- C2258-90M-BIOTIN
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Rat thymocyte membrane glycoproteins
- Clone:
- OX-8
- Format:
- Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
- Extra Details:
- Applications: Suitable for use in Flow Cytometry, in vitro blocking studies, and Immunohistochemistry (frozen, paraffin-embedded following PLP Fixation (periodate-lysine paraformaldehyde). Other applications not tested. Recommended Dilution: Flow Cytometry: 1:100 Use 10ul to label 10e6 cells in 100ul. Immunohistochemistry: Does not require protein digestion pre-treatment of paraffin embedded sections, Does not require antigen retrieval using heat treatment prior to staining of paraffin embedded sections. Optimal dilution determined by the researcher Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the NS1 mouse myeloma cell line. Recommended Secondary Reagents: IgG Goat anti mouse IgG:FITC (rat adsorbed) IgG Goat anti mouse IgG:RPE (rat adsorbed) Recommended Negative Controls: Mouse IgG1 Negative Control Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.
- Shipping Conditions:
- Blue Ice