C2257-01D
CD6
Size
£479.00
- SKU:
- C2257-01D
- Purity:
- Purified by Protein A affinity chromatography from tissue culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human rheumatoid synovial T cell line (ST-1)
- Clone:
- 7H122
- Format:
- Supplied as a liquid in PBS, pH 7.5, 0.5mg/ml gentamicin sulfate.
- Extra Details:
- Human CD6 is expressed on peripheral blood T cells, most thymocytes and a subset of B cells. CD6 is similar to CD5 and contains three cysteine-rich domains with structural homology to the macrophage scavenger receptor. CD6 is involved in T cell activation and its ligand was recently identified. Antibody is a useful reagent to study T cell activation through CD6. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Immunohistochemistry: Frozen sections Flow Cytometry: 10ug/ml Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Five x 10e5 ficoll prepared human peripheral blood mononuclear cells per tube were washed and incubated 45 minutes on ice with 80ul of C2257-01D at 10ug/ml. 2. Cells were washed twice and incubated with goat anti-mouse IgG (FITC). 3. Cells were washed three times, fixed and analyzed by FACS. 4. An 82% sub population of cells stained positivewith a mean shift of 1.34 log10 fluorescent units when compared to a mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice