C2256-65E-BIOTIN

CD5L, CT (CD5 Antigen-like, CD5 Molecule-like, AIM, API6, CT-2, IgM-associated Peptide, PRO229, SPalpha, SP-alpha) (Biotin)

Size

£869.00

SKU:: C2256-65E-BIOTIN
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 13aa near the C-terminus of human CD5L. Epitope: aa250-300
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: CD5L has been shown to mediate the survival of macrophages in atherosclerotic plaques. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: detects a band of ~38kD in Raji cell lysates. Immunohistochemistry (Paraffin): Requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer, pH 6.0 is recommended. Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.