C2256-23L

CD5 (Lymphocyte Antigen T1/Leu 1, p56 62, T cell Surface Glycoprotein CD5, T1) (Biotin)

Size

£544.00

SKU:: C2256-23L
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Clone:: 4H8E5
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
Extra Details:: CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells.1-3 It has been identified as the major ligand of the B-cell antigen CD72.4, 5 The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in 6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression.7-12 It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.13, 14 Applications: • Identification and enumeration of CD5+ cells by flow cytometry 15 • Immunoprecipitation 15 • Immunohistochemistry (frozen sections) 15 • T cell activation 15 • Western blotting 15 Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice