C2256-23B

CD5 (Lymphocyte Antigen T1/Leu 1, p56 62, T cell Surface Glycoprotein CD5, T1) (Biotin)

Size

£429.00

SKU:: C2256-23B
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Clone:: B19.1
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Biotin.
Extra Details:: CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells (1-3). It has been identified as the major ligand of the B-cell antigen CD72.4, 5 The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in 6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression.7-12 It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.13, 14 Applications: • Identification and enumeration of CD5+ cells by flow cytometry 15 • Identification of CD4+ cells in acetone-fixed frozen sections 15 • Immunoprecipitation 15 • Activation of T cells in vitro 15 • Western blotting 15 Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice