C2256-23A

CD5 (Lymphocyte Antigen T1/Leu 1, p56 62, T cell Surface Glycoprotein CD5, T1) (FITC)

Size

£314.00

SKU:: C2256-23A
Application:: Flow Cytometry
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Clone:: B19.1
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
Extra Details:: CD5/Lyt-1 antigen is a monomeric type I transmembrane glycoprotein expressed on thymocytes, T lymphocytes, and a subset of B lymphocytes, but not on natural killer (NK) cells (1-3). It has been identified as the major ligand of the B-cell antigen CD72.4, 5 The frequency of CD5+ B cells exhibits strain-dependent variation, and the phenotypic, anatomical, functional, developmental, and pathological characteristics of the CD5+ B cells suggest that they may represent a distinct lineage, known as B-1 cells (reviewed in 6). Binding of CD5 on the T cell surface can augment alloantigen- or mitogen-induced lymphocyte proliferation and induces increased cytosolic free calcium, IL-2 secretion, and IL-2R expression.7-12 It has been proposed that CD5 negatively regulates signal transduction mediated by the T-cell and B-cell receptors.13, 14 Applications: Suitable for use in Flow Cytometry. Though not tested, Western Blot and Immunoprecipitation have been reported in literature. See ref 5. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice