C2255-31B
CD4 (L3T4) (Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (FITC)
Size
£314.00
- SKU:
- C2255-31B
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Cloned mouse CTL line V41
- Clone:
- 5K206 (GK1.5)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- CD4/L3T4 is a type I transmembrane glycoprotein expressed on most thymocytes and the helper/inducer subpopulation of mature T lymphocytes of all mouse strains tested (1,2). CD4 has also been detected at low density on pluripotent hematopoietic stem cells, bone marrow myeloid precursors, and intrathymic lymphoid precursors (3-6). The CD4 antigen functions as a co-receptor, interacting with class II major histocompatability complex (MHC) molecules in the recognition of foreign antigens by T cells (1,2). In vivo and in vitro treatment with the effectively depletes CD4+ cells (7,8). The mAb also blocks helper T cell responses to MHC class II antigens, including cytolysis, proliferation, allogeneic B cell help, and release of lymphokines (1,8-13). Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunoprecipitation, In vivo and in vitro depletion of CD4+ cells, Inhibition of T helper function and in Fractionation of CD4+ cells. Other applications have not been tested. Recommended Dilution: Flow Cytometry: ≤1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice