C2255-28K

CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (FITC)

Size

£620.00

SKU:: C2255-28K
Application:: Flow Cytometry
Purity:: Purified by Protein G affinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human PHA blasts.
Clone:: RPA-T4
Format:: Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
Extra Details:: CD4 is a 55kD cell surface glycoprotein that is primarily expressed on a subpopulation of T lymphocytes, on peripheral blood monocytes and on tissue macrophages. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: Neat. Use 10ul to label 10e6 cells or 100ul whole blood. Optimal working dilutions to be determined by researcher. Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line. Recommended Negative Controls: I1904-78R3 IgG1 Murine Negative Control (FITC) Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.