C2255-27G
CD4, Recombinant, Human (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4
Size
£632.00
- SKU:
- C2255-27G
- Molecular Weight:
- 55
- Purity:
- Purified by immunoaffinity and gel exclusion chromatography to ~95% purity as determined by SDS PAGE, reduced.
- Storage Conditions:
- -70°C
- Supplier:
- United States Biological
- Format:
- Supplied as a liquid in PBS, pH 7.2. No stabilizers or preservatives added.
- Extra Details:
- Full length recombinant human T-Cell receptor sCD4 produced in the baculovirus expression system. The full length protein contains V1 through V3 domains of CD4. Specificity: This protein binds to murine monoclonal antibodies of defined epitope specificity in ELISA and Western Blot. Biological Activity: This protein binds to native and recombinant HIV-1 gp120 and HIV-2 gp105 and neutralizes HIV-1 infectivity in CD4-bearing, HIV-1 infected cell cultures and HIV infected PCMBs. Applications: Suitable for use in ELISA, Neutralization, Drug Screening and Western Blot. Other applications not tested. Recommended Dilutions: ELISA: 10-100ng protein depending on the nature and affinity of the secondary detector reagent(s). Western Blot: 10-100ng protein depending on the nature and affinity of the secondary detector reagent(s). Neutralization: 1-10ug/ml of the HIV-1 infected culture. Drug screening assays: 100-500ng of solid phase sCD4 and gp120 binding is determined in the presence of inhibitors either indirectly using H6003-34G1, HIV-1 gp120 (HRP) or directly using H6003-07B1 HIV-1 env gp120, Strain IIIB, Recombinant (HRP) Optimal dilution determined by the researcher Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Dry Ice