C2255-05B-BIOTIN

CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4, L3T4, LY-4, Tac antigen, p55, Interleukin 2 Receptor alpha, IL2Ra, T Cell Growth Factor Receptor) (Biotin)

Size

£837.00

SKU:: C2255-05B-BIOTIN
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Mouse CD4
Clone:: 10A3 (GK1.5)
Format:: Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Extra Details:: Recognises mouse CD4, a 55 kDa protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+ CD25+), which play a key role in the maintenance of self tolerance. Clone GK1.5 has been reported to block CD4+ T-cell activation. It blocks class II MHC antigen-specific binding, thereby inhibiting functions such as class II MHC antigen-specific proliferation and the release of lymphokines. It may also be used for in vivo and in vitro cell depletion of CD4+ T-cells. Applications: Suitable for use in Immunofluorescence, Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with Sspleen cells from immunised Lewis rats. Recommended Negative Control: I1904-82V4 IgG2b, Rat Negative Control I1904-82V3 IgG2b, Rat Negative Control I1904-82V2 IgG2b, Rat Negative Control (Azide Free) I1904-82V1 IgG2b, Rat Negative Control (Biotin) Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Note: Applications are based on unconjugated antibody.

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