C2255-03H
CD4 (CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Surface Glycoprotein CD4) (Biotin)
Size
£557.00
- SKU:
- C2255-03H
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- CD4+ transfectant/human CEM
- Clone:
- 7H214
- Format:
- Supplied as a liquid in PBS, pH 7.5, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with Biotin.
- Extra Details:
- Human CD4 is a cell surface glycoprotein of 55kD expressed on most thymocytes, on about two thirds of peripheral T cells, and on some monocyte macrophage lineage cells. CD4 interacts with MHC Class II molecules in an accessory role during foreign antigen recognition by T cells. The extracellular portion of CD4 is an array of four Ig-like domains. The amino terminal region of CD4 is a receptor for the HIV-1 viral protein gp120. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Functional applications. Other applications not tested. Recommended Dilution: Flow Cytometry: 5ug/ml. Protocol: 5x10e5 cultured CEM cells were washed and incubated 45 min. on ice with 80ul of C2255-03H at 5ug/ml. Cells were then washed 2X and incubated 45 min. with 20ul of secondary reagent (Streptavidin(PE), S7973-78). Cells were washed 3X, fixed and analyzed. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice