C2255-02N

CD4 (CD4 Antigen, CD4 Molecule, CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Antigen T4, T Cell Surface Glycoprotein CD4)

Size

£691.00

SKU:: C2255-02N
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: BALB/c mouse thymocytes.
Clone:: RM4-5
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: CD4 is a 55 kD protein also known as Ly-4 and L3T4. CD4 is a single chain transmembraneous glycoprotein which belongs to the immunoglobulin superfamily, and is primarily expressed on peripheral blood monocytes and tissue macrophages. CD4 is also expressed on a subpopulation of regulatory T cells (CD4+CD25+), which play a key role in the maintenance of self tolerance. Applications: Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells in 100ul Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-50U1: IgG H&L, X-adsorbed (HRP) Pab Gt xRt I1904-47N: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRt Recommended Negative Control: I1904-82D1: IgG2a, Rat Negative Control Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.