C2254-92M
CD4 (T-Cell Surface Antigen T4/Leu-3, T-Cell Surface Glycoprotein CD4) (APC)
Size
£767.00
- SKU:
- C2254-92M
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- P815 cell transfected with human CD4
- Clone:
- M-T441
- Format:
- Supplied as a liquid in 50mM sodium phosphate pH 7.5, 500mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 15% glycerol. Labeled with allophycocyanin (APC).
- Extra Details:
- Human CD4 is a cell surface glycoprotein of 55kD expressed on most thymocytes, on about two thirds of peripheral T cells, and on some monocyte macrophage lineage cells. CD4 interacts with MHC Class II molecules in an accessory role during foreign antigen recognition by T cells. The extracellular portion of CD4 is an array of four Ig-like domains. The amino terminal region of CD4 is a receptor for the HIV-1 viral protein gp120. Applications: Suitable for use in FLISA and Flow Cytometry. Other applications not tested. Recommended Dilution: FLISA: Detector Flow Cytometry: 1:50 - 5x10e5 ficoll prepared human PBMC were washed and pre-incubated on ice with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 min on ice with 80ul of anti-CD4/APC at a 1:50 dilution (5ug/ml). Cells were washed three times, fixed and analyzed by FACS. A 52% sub population of cells stained positive with a mean shift of 2.0 log10 fluorescent units when compared to a buffer background. Binding was blocked when cells were pre-incubated 10 min with 20ul of 0.5mg/ml anti-CD4 antibody. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.
- Shipping Conditions:
- Blue Ice