C2254-92L
CD4 (T-Cell Surface Antigen T4/Leu-3, T-Cell Surface Glycoprotein CD4)
Size
£479.00
- SKU:
- C2254-92L
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- P815 cell transfected with human CD4
- Clone:
- M-T441
- Format:
- Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride. No preservative added.
- Extra Details:
- Human CD4 is a cell surface glycoprotein of 55kD expressed on most thymocytes, on about two thirds of peripheral T cells, and on some monocyte macrophage lineage cells. CD4 interacts with MHC Class II molecules in an accessory role during foreign antigen recognition by T cells. The extracellular portion of CD4 is an array of four Ig-like domains. The amino terminal region of CD4 is a receptor for the HIV-1 viral protein gp120. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilution: ELISA: Detector Flow Cytometry: 5x10e5 cultured HPB-MLT cells were washed and incubated 45 min on ice with 80ul of anti-CD4 antibody at 5ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.68 log10 fluorescent units when compared to a Mouse IgG2b negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice