C2254-68B
CD56 (MSK39, NCAM, NCAM-1, N-CAM-1, Neural cell adhesion molecule 1)
Size
£479.00
- SKU:
- C2254-68B
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human retinoblastoma tumor tissue, membrane fraction
- Clone:
- ERIC-1
- Format:
- Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100mM potassium chloride, 150mM sodium chloride.
- Extra Details:
- Human CD56 is an adhesion molecule from the Ig superfamily which is restricted to NK cells in the immune system. It is believed that NK cells form a first line of defense against tumor cells and cells infected with bacteria and viruses. Applications: Suitable for use in Flow Cytometry, Western Blot, ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry: Acetone fixation Flow Cytometry: 80ul (10ug/ml) to label 5x10e5 human peripheral blood mononuclear cells Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. 5x10e5 ficoll prepared human peripheral blood mononuclear cells were washed and pre-incubated for 5 minutes with 20ul of 250ug/ml human IgG (to block non-specific binding) after which they were incubated 45 minutes on ice with 80ul of C2254-68B at 10ug/ml. 2. Cells were washed twice and incubated 2º with IgG (FITC) goat anti-mouse, after which they were washed three times, fixed and analyzed by FACS. 3. A 15% sub population of the cells stained positive with a mean shift of 0.91 log10 fluorescent units when compared to a mouse IgG1 negative control at a similar concentration. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice