C2254-56Q

CD3 (Cy5)

Size

£512.00

SKU:: C2254-56Q
Application:: Flow Cytometry
Purity:: Purified
Storage Conditions:: 4°C Do not freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human thymocytes and peripheral blood lymphocytes from a patient with Sezary cell leukemia.
Clone:: 5K205 (UCHT1)
Format:: Supplied as a liquid in PBS, 0.09% sodium azide. Labeled with Cy5.
Extra Details:: CD3, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging from 16-28kD and is closely associated with the T cell antigen receptor (TCR). CD3 is expressed on 70-80% of normal peripheral blood lymphocytes and on 10-20% of thymocytes. It plays a major role in signaling during antigen recognition, leading to T cell activation. Applications: • Flow cytometry • Immunohistochemistry (frozen sections) • Immunoprecipitation • In vitro activation Recommended Dilution: Flow Cytometry: 10ul/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.