C2254-56D
CD3
Size
£576.00
- SKU:
- C2254-56D
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human thymocytes and T lymphocyte
- Clone:
- MEM-57
- Format:
- Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
- Extra Details:
- These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Immunoprecipitates from a detergent lysate of surface-radioiodinated T cells a strong zone of about 22kD and a weak 28kD zone, which is typical pattern yielded by a reference antibody Leu-4 (SK7). Applications: Suitable for use in Immunoprecipitation and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice