C2254-39H
CD3 (Early T Cell Marker, T Cell receptor Complex, TCR) (PE)
Size
£730.00
- SKU:
- C2254-39H
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome.
- Clone:
- UCHT1
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.2, 0.09% sodium azide, 1% BSA. Labeled with R. Phycoerythrin (RPE). Reconstitute with 1ml sterile 40-50% glycerol, ddH2O.
- Extra Details:
- CD3 is closely associated on the lymphocyte cell surface with the T cell antigen receptor TcR (2-4). It is believed that the CD3 complex transmits activational signals to the T cell’s interior upon antigen recognition by TcR. CD45 (LCA), a protein tyrosine phosphatase, is a potent regulator of signal transduction by the CD3 complex. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukemia cells, Sézary leukemias and approximately 70% of acute lymphoblastic leukemias of T cell origin. Applications: Flow Cytometry: Neat. Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood. Optimal working dilutions to be determined by researcher. Recommended Negative Controls: Mouse IgG1 Negative Control: RPE Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice