C2254-38X
CD3 (Early T Cell Marker, T Cell receptor Complex, TCR) (FITC)
Size
£620.00
- SKU:
- C2254-38X
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- 4°C (-20°C Glycerol)
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Human infant thymocytes and lymphocytes from a patient with Sezary Syndrome.
- Clone:
- 3H701 (UCHT1)
- Format:
- Supplied as a liquid in PBS, pH 7.2, 1% BSA, 0.09% sodium azide. Labeled with FITC.
- Extra Details:
- CD3 is closely associated on the lymphocyte cell surface with the T cell antigen receptor TcR (2-4). It is believed that the CD3 complex transmits activational signals to the T cell’s interior upon antigen recognition by TcR. CD45 (LCA), a protein tyrosine phosphatase, is a potent regulator of signal transduction by the CD3 complex. It reacts with the majority of peripheral blood T lymphocytes, a major proportion of thymocytes, the majority of T cell chronic lymphocytic leukemia cells, Sézary leukemias and approximately 70% of acute lymphoblastic leukemias of T cell origin. Applications: Suitable for use in Flow Cytometry. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:10 Use 10ul of the suggested working dilution to label 10e6 cells or 100ul whole blood. Optimal working dilutions to be determined by researcher. Hybridoma: Spleen cells from immunized BALB/c mice were fused with cells of the P3/NS1/1-Ag4-1 mouse myeloma cell line. Recommended Negative Controls: I1904-78R3: Mouse IgG1 Negative Control: FITC Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice