C2254-38R

CD3 (T cell antigen receptor complex, T3) (Azide Free)

Size

£576.00

SKU:: C2254-38R
Application:: Flow Cytometry
Purity:: Purified by affinity chromatography. Endotoxin: ≤0.01EU/µg (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: F344 rat spleen cells stimulated with PMA and calcium ionophore
Clone:: 1F4
Format:: Supplied as a liquid in PBS, pH 7.2. No stabilizing proteins added. No preservative added.
Extra Details:: CD3 is a complex composed of δ, γ, ε, and ζ chains. They are 20-25 kD members of the immunoglobulin superfamily and associated with the T cell receptor (TCR). CD3 is expressed on thymocytes, peripheral T cells, some NK-T cells, and dendritic epidermal T cells. CD3 is involved in antigen recognition, signal transduction, and T cell activation. Applications: Suitable for use in Flow Cytometry. Though not tested Immunohistochemistry has been reported in literature. Other applications not tested. Recommended Dilutions: Flow Cytometry: ≤ 0.25ug labels 10e6 cells in 100ul Optimal working dilutions to be determined by researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.