C2254-38J

CD3 (Early T Cell Marker, T Cell receptor Complex, TCR)

Size

£563.00

SKU:: C2254-38J
Purity:: Purified by ammonium sulfate precipitation from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: F344 rat T cells stimulated with PMA (TPA) and calcium ionophore.
Clone:: 1F4
Format:: Supplied as a liquid in PBS, 0.09% sodium azide.
Extra Details:: Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. In addition of this role of signal transduction in T-cell activation, CD3D plays an essential role in thymocyte differentiation. Indeed, participates in correct intracellular TCR-CD3 complex assembly and surface expression. In absence of a functional TCR-CD3 complex, thymocytes are unable to differentiate properly. Interacts with CD4 and CD8 and thus serves to establish a functional link between the TCR and coreceptors CD4 and CD8, which is needed for activation and positive selection of CD4 or CD8 T-cells. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 1:10-1:25. 10ul labels 10e6 cells in 100ul. Immunohistochemistry (frozen): 1:10-1:25 Immunohistochemistry (paraffin): requires antigen retreval Optimal working dilutions to be determined by researcher. Recommended Secondary Reagents: Recommended Secondary Antibodies: I1905-18D: IgM (X-Adsorbed) (FITC) Pab GtxMo I1905-04A: IgM (X-Adsorbed) (PE) Pab GtxMo I1905-28A: IgM (X-Adsorbed) (HRP) Pab GtxMo Hybridoma: P3X63-Ag8.653 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice