C2254-18G

CD3e (T-cell Surface Glycoprotein CD3 epsilon Chain, T-cell Surface Antigen T3/Leu-4 epsilon Chain, AI504783, CD3epsilon, T3e)

Size

£288.00

SKU:: C2254-18G
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Hamster
Immunogen:: H-2Kb-specific mouse cytotoxic T lymphocyte
Clone:: 145-2C11
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: CD3e is a 20kD transmembrane protein, also known as CD3 or T3. It is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3e forms a TCR complex by associating with the CD3d, g and z chains, as well as the TCR a/b or g/d chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry, Immunofluorescence, Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤1ug/10e6 cells in 100ul volume Immunohistochemistry (frozen tissue sections): 5-10ug/ml. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.