C2254-18B

CD3e (Early T Cell Marker)

Size

£1,023.00

SKU:: C2254-18B
Purity:: Culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified CD3εγδ/CD3ω’. Epitope: Cytoplasmic region of e chain. Cellular Localization: Cell membrane
Clone:: F7.2.38
Format:: Supplied as a liquid in 0.09% sodium azide.
Extra Details:: CD3 consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta). The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). It is involved in signal transduction to the T cell interior following antigen recognition. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms. Applications: Suitable for use in ELISA and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (FFPE): 1:10-1:20 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 20 min followed by cooling at RT for 20 min. Optimal dilution determined by the researcher. Positive Control: Tonsil Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.