C2254-11N
CD3, epsilon (Early T Cell Marker) (Biotin)
Size
£416.00
- SKU:
- C2254-11N
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- IL-4 producing Th2 cell lines including D10
- Clone:
- C363.29B (YCD3-1)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Biotin.
- Extra Details:
- CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains (1,2). CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. In the presence of Fc receptor-bearing accessory cells, soluble antibody can activate primed and naïve T cell in vitro. Immobilized monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present (1,3,4). The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody (1). Applications: • Identification and enumeration of CD3+ cells by flow cytometry 1, 2 • Immunoprecipitation 1 • In vitro depletion of CD3+ cells 1 • In vitro activation of T cells (accessory cells must be provided) 1, 3, 4 • Immunohistochemistry 5 Recommended Dilution: Flow Cytometry: ≤ 3ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice