C2254-11M
CD3, epsilon (Early T Cell Marker) (FITC)
Size
£282.00
- SKU:
- C2254-11M
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- IL-4 producing Th2 cell lines including D10
- Clone:
- C363.29B (YCD3-1) (5K4)
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. In the presence of Fc receptor-bearing accessory cells, soluble antibody can activate primed and naïve T cell in vitro. Immobilized monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Flow Cytometry: ≤3ug/10e6 cells Immunohistochemistry: Paraffin, frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice