C2254-11L
CD3, epsilon (Early T Cell Marker)
Size
£364.00
- SKU:
- C2254-11L
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- IL-4 producing Th2 cell lines including D10
- Clone:
- C363.29B (YCD3-1)
- Format:
- Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
- Extra Details:
- CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains (1,2). CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. In the presence of Fc receptor-bearing accessory cells, soluble monoclonal antibody can activate primed and naïve T cell in vitro. Immobilized monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present (1,3,4). The monoclonal antibody is cytolytic, easily used for cell surface staining and a good immunoprecipitating antibody (1). Applications: Suitable for use in Flow Cytometry, Immunoprecipitation, Immunohistochemistry, In vitro depletion of CD3+ cells and In vitro activation of T cells (accessory cells must be provided). Other applications have not been tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice