C2254-11H

CD3, epsilon (Early T Cell Marker) (APC)

Size

£448.00

SKU:: C2254-11H
Purity:: Purified
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Hamster
Immunogen:: T cell receptor complexes derived from C6VL-BS thymoma cells (C57BL/Ka)
Clone:: 500-A2
Format:: Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide and a stabilizing agent. Labeled with APC.
Extra Details:: CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). CD3e is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. Applications: • Identification and enumeration of CD3+ cells by flow cytometry 1 • Immunoprecipitation 2 • In vitro activation of T cells 2, 3, 4 Recommended Dilution: Flow Cytometry: ≤ 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for at least 12 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.