C2254-11D

CD3, epsilon (Early T Cell Marker)

Size

£364.00

SKU:: C2254-11D
Purity:: Purified
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Hamster
Immunogen:: T cell receptor complexes derived from C6VL-BS thymoma cells (C57BL/Ka)
Clone:: 500-A2
Format:: Supplied as a liquid in 100mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added.
Extra Details:: CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). CD3e is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. Applications: • Identification and enumeration of CD3+ cells by flow cytometry 1 • Immunoprecipitation 2 • In vitro activation of T cells 2, 3, 4 Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.