C2254-10J
CD3, epsilon (Early T Cell Marker) (PE)
Size
£338.00
- SKU:
- C2254-10J
- Purity:
- Purified
- Storage Conditions:
- 4°C Do not freeze
- Supplier:
- United States Biological
- Host:
- Hamster
- Immunogen:
- H-2Kb-specific mouse cytotoxic T lymphocyte
- Clone:
- 145-2C11
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide, stabilizing agent. Labeled with R-Phycoerythrin (PE).
- Extra Details:
- CD3, epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. In the presence of Fc receptor-bearing accessory cells, soluble antibody can activate primed and naïve T cell in vitro. It can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL’s. Immobilized antibody can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by this antibody may result in apoptotic cell death (1-6). Applications: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, In vitro depletion of CD3+ cells and In vitro activation of T cells. Other applications not tested. Recommended Dilution: Flow Cytometry: ≤0.1ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Dilute only prior to immediate use. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.
- Shipping Conditions:
- Blue Ice