C2254-10G
CD3, epsilon (Early T Cell Marker) (FITC)
Size
£282.00
- SKU:
- C2254-10G
- Purity:
- Purified
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Hamster
- Immunogen:
- H-2Kb-specific mouse cytotoxic T lymphocyte clone BM10-37
- Clone:
- 145-2C11
- Format:
- Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide. Labeled with FITC.
- Extra Details:
- CD3epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chains ranging in size from 16-28kD and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. In the presence of Fc receptor-bearing accessory cells, soluble antibody can activate primed and naïve T cell in vitro. It can also induce redirected lysis of specific target cells by CTL clones and it can block lysis of specific target cells by antigen-specific CTL’s. Immobilized antibody can activate both normal T lymphocytes and cloned T cell lines. Under certain conditions, T cell activation by this antibody may result in apoptotic cell death (1-6). Applications: • Identification and enumeration of CD3+ cells by flow cytometry 1, 2 • Immunoprecipitation 1 • Western blotting 7 • In vitro depletion of CD3+ cells 2 • In vitro activation of T cells 2-4 • Immunohistochemistry 8 Recommended Dilution: Flow Cytometry: ≤ 3ug/10e6 cells Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice