C2253-38A

CD3, epsilon (Early T Cell Marker)

Size

£563.00

SKU:: C2253-38A
Purity:: Purified by Protein G affinity chromatography from tissue culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Hamster
Immunogen:: H-2Kb-specific mouse cytotoxic T lymphocyte.
Clone:: 145-2C11
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: CD3 epsilon, a 20kD transmembrane protein, is a member of the CD3 complex which consists of four subunits, gamma, delta, epsilon and zeta, that are associated to the T cell receptor (TCR). TCR plays a critical role in T cell development and function, and is responsible for ligand recognition. It interacts non-covalently with the CD3 dimers delta/epsilon, gamma/epsilon and zeta/zeta which transduce signals from the TCR into the cell. Applications: Suitable for use in Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:25-1:200; 10ul labels 1x10e6 cells in 100ul Immunohistochemistry: frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.