C2253-01T

CD2 (Erythrocyte Receptor, FLJ46032, LFA-2, LFA-3 Receptor, Rosette Receptor, SRBC, T11, T-Cell Surface Antigen CD2, T-Cell Surface Antigen T11/Leu-5)

Size

£479.00

SKU:: C2253-01T
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Human rheumatoid synovial T cell line (ST-1)
Clone:: UMCD2/IE7E8
Format:: Supplied as a liquid in 50mM sodium phosphate, pH 7.5, 100,mM potassium chloride, 150mM sodium chloride.
Extra Details:: Human CD2 is a T-lineage glycoprotein with roles in cell-cell adhesion and the transduction of activation signals. Multiple ligands for CD2 include CD58 (LFA-3), CD59, and CD48. CD2 may play a role in regulating T cell anergy. Applications: Suitable for use in Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 5x10e5 cultured HPB-MLT human tumor cells were washed and incubated 45 min on ice with 80ul anti-CD2 antibody at 1ug/ml. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.12 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.