C2099-81D
CCRL2 (C-C Chemokine Receptor-like 2, Chemokine Receptor CCR11, Chemokine Receptor X, Putative MCP-1 Chemokine Receptor, CCR11, CCR6, CKRX, CRAM, HCR) (APC)
Size
£812.00
- SKU:
- C2099-81D
- Application:
- Flow Cytometry
- Purity:
- Purified by Protein A affinity chromatography from hybridoma culture supernatant
- Storage Conditions:
- 4°C Do Not Freeze
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant protein corresponding to Met1-Val356 (Val180Met) of human CCRL2 expressed in NS0 cell line.
- Clone:
- 12K19
- Format:
- Supplied as a liquid in saline, 0.5% BSA, 0.09% sodium azide. Labeled with Allophycocyanin (APC).
- Extra Details:
- CCRL2, also known as CRAM-A, CRAM-B, CKRX, and HCR, is a seven transmembrane G protein-linked receptor that shares homology with other human chemokine receptors. Two isoforms were reported and designated CRAM-A and CRAM-B that differ at their N-termini by the inclusion of an additional 12aa in CRAM-A. CCRL2 is expressed at varying levels on a variety of peripheral blood cells, including monocytes, neutrophils, and T cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul/10e6 cells. Validated using human PBMCs cultured with Recombinant Human IL-2. Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1.Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction. 2.After blocking, add 10ul of C2099-81D to up to 1 x 10e6 cells and incubated for 30 minutes at RT. 3.Unbound antibody was removed by washing the cells twice in Flow Cytometry Staining Buffer (Catalog# F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer. 4. Resuspend the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled mouse IgG2a antibody. Excitation: 620-650nm Emission: 660-670nm Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
- Shipping Conditions:
- Blue Ice