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C2099-81C

CCRL2 (Chemokine (C-C motif) Receptor-Like 2)

Size

£812.00

SKU:
C2099-81C
Purity:
Purified by Protein G affinity chromatography from hybridoma culture supernatant
Storage Conditions:
-20°C
Supplier:
United States Biological
Host:
Rat
Immunogen:
RNL-1 rat basophilic leukemia cell line transfected with mouse CCRL2/LCCR. Species Sequence Homology: human, 49% and rat, 70%.
Clone:
11n20
Format:
Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:
Lipopolysaccharide-inducible CC chemokine receptor (LCCR) is a 7TM chemokine receptor-like protein that is expressed on macrophages, glial cells, and mast cells at inflammatory sites. LCCR functions as a receptor for Chemerin, CCL2, 5, 7, and 8. Mouse LCCR shares 49%, 70% aa sequence identity with human and rat LCCR, respectively. Applications: Suitable for use in Flow Cytometry and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 cells. Using RAW 264.7 mouse monocyte/macrophage cell line Immunocytochemistry: 8-25ug/ml. Using immersion fixed RAW264.7 mouse monocyte/macrophage cell line Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Shipping Conditions:
Blue Ice