C2099-71M

CCR7 (Chemokine Receptor 7, CC-CCR-7)

Size

£908.00

SKU:: C2099-71M
Purity:: Purified by Protein G affinity chromatography. Endotoxin: <0.1EU/ug (LAL)
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant corresponding to mouse CCR7 expressed in RBC-2H3 cells (NP_031745).
Clone:: 8H212
Format:: Supplied as a lyophilized powder in PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: CCR7 is a 7TM G protein coupled chemokine receptor. CCR7 is expressed on T cells and mature dendritic cells and transduces chemotactic signals in response to CCL19 and CCL21. Mouse CCR7 shares 87% amino acid sequence identity with human CCR7. Applications: Suitable for use in Flow Cytometry, Immunohistochemistry and Neutralization. Other applications have not been tested. Recommended Dilution: Flow Cytometry: 25ug/ml; 10ul of the diluted solution labels 1-2.5x10e5 cells in a total volume ≤ 200ul. Immunohistochemistry: 25ug/ml stains paraformaldehyde-fixed mouse splenocytes. Neutralization: The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. The ND50 for this lot of C2099-71M was determined to be approximately 2-10ug/ml in the presence of 25ng/ml of rmMIP-3beta using the BaF/3 mCCR7 cell line in a chemoattraction assay. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice