C2099-60
CCL26-Like (CCL26L, Eotaxin-3 Like)
Size
£850.00
- SKU:
- C2099-60
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- E. coli-derived recombinant rat CCL26-like/Eotaxin‑3-like, Thr24-Trp94.
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Chemokine (CC motif) ligand 26, also known as eotaxin 3, is a human CC chemokine ligand that binds and signals via the chemokine receptor CCR3. Rat CCL26-like/Eotaxin 3-like is an 8.5kD orthologue of the human CCL26/eotaxin 3. It is a 94 amino acid (aa) residue protein with a 23 aa signal sequence and a 71 aa mature segment. It is presumed that rat CCL26-like/Eotaxin 3-like will bind CCR3 to induce eosinophil chemotaxis and transendothelial migration. Mature rat CCL26 shares 48%, 63%, and 48% aa sequence identity with the human, mouse and canine CCL26/Eotaxin 3, respectively. Applications: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect rat CCL26-like. The detection limit for rrCCL26-like is approximately 1 ng/lane under non-reducing and reducing conditions, respectively. Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect rat CCL26-like. The detection limit for rrCCL26-like is approximately 0.5ng/well. Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice