CCCTC-Binding Factor (CTCF, CTCFL Paralog, 11-Zinc Finger Protein)

Size

£863.00

SKU:: C2099-05G
Application:: WB
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Sheep
Immunogen:: Recombinant protein corresponding to Met1-Ile154 from human CTCF expressed in E. coli.
Format:: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Extra Details:: CTCF (CCCTC-binding factor) is a 130-140kD member of the CTCF Zn-finger protein family. Although its predicted MW is 83kD, it runs anomalously slow in SDS-PAGE. CTCF is ubiquitously expressed, save for primary spermatocytes, and serves multiple functions. It regulates both gene activation and repression by binding to unmethylated DNA sequences. Structurally, it contains eleven Zn-finger motifs, some or all of which may be involved in DNA binding. This variation in number allows for different conformations and multiple interaction partners. Human CTCF is 727 aa in length. It contains two SUMOylation sites (Lys74 and Lys689) and eleven consecutive C2H2-type Zn-finger domains (aa266-577). There are multiple Ser/Thr phosphorylation sites. Two potential alternative start sites exist; one nine aa upstream of thestandard start site, and another at Met570. A third splice variant shows a 62aa substitution for (aa349-727). Over (aa1-154), human CTCF shares 99% aa identity with mouse CTCF. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml using MOLT-4 human acute lymphoblastic leukemia cell line and Jurkat human acute T cell leukemia cell line. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice