C2098-03X

CBP, p300 (CREB Binding Protein, Histone Acetyl Transferase, RTS, RSTS, Rubinstein-Taybi Syndrome) (BSA & Azide Free)

Size

£1,144.00

SKU:: C2098-03X
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: E1A affinity purified p300 protein. Cellular Localization; Nuclear. MW of Antigen; 300kD. Epitope; aa2071-2091.
Clone:: 3F310
Format:: Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See C2098-03.
Extra Details:: p300 is a transcriptional adapter protein which is characterized by three cysteine-and histidine-rich regions and its C-terminus specifically binds the adenovirus E1A protein. p300, and p300-associated proteins, are components of TATAbinding protein (TBP) complexes. Protein kinase A mediated CREB phosphorylation results in the binding of CREB to a 265kD nuclear protein designated CBP (for CREB-binding protein). CBP and p300 are homologous to each other. Applications: Suitable for use in Immunofluorescence and Immunohistochemistry. Not suitable for use in Western Blot. Other applications have not been tested. Recommended Dilution: Immunohistochemistry (Formalin/paraffin): 1:50-1:100 for 30 min at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilutions to be determined by the researcher Positive Control: Tonsil Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice