C2097-91Q

Caveolin 1 (CAV1, Caveolae protein, HGNC:1527, CAV, MSTP085, Cell Growth-inhibiting Protein 32, VIP21)

Size

£754.00

SKU:: C2097-91Q
Purity:: Purified by Protein A affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Intracellular membrane protein-containing vesicles (containing GLUT4) from rat adipocytes
Clone:: 8J542 (7C8)
Format:: Supplied as a liquid in Tris-glycine, 150mM sodium chloride, 0.05% sodium azide.
Extra Details:: Caveolae are specialized domains of the plasma membrane that are implicated in the sequestration of a variety of lipid and protein molecules. It has been suggested that these important cellular organelles have a pivotal role in such diverse biochemical processes as lipid metabolism, growth regulation, signal transduction, and apoptosis. Caveolin interacts with and regulates heterotrimeric G-proteins. Currently, there are three members of the caveolin multigene family which are known to encode 21-24kD integral membrane proteins that comprise the major structural component of the caveolar membrane in vivo. Caveolin-2 protein is abundantly expressed in fibroblasts and differentiated adipocytes, smooth and skeletal muscle, and endothelial cells. The expression of caveolin-1 is similar to that of caveolin-2 while caveolin-3 expression appears to be limited to muscle tissue types. Applications: Suitable for use in Flow Cytometry, Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:4000 Immunocytochemistry (IF): 1:200 Immunohistochemistry (Paraffin): 1:100-1:300 Immunohistochemistry: Frozen sections Immunoprecipitation: 1-2ug/500ug protein Flow Cytometry: 1ug/10e6 cells Optimal dilutions to be determined by the researcher. Positive Control: A431 and 3T3 whole cell lysates Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice