C2097-90C
Caveolin 1 (Caveolin-1, CAV 1, CAV1, CAV-1, CAV, Caveolae Protein 22kD, Cell Growth-inhibiting Protein 32)
Size
£863.00
- SKU:
- C2097-90C
- Application:
- WB
- Purity:
- Purified by immunoaffinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Goat
- Immunogen:
- E.coli-derived recombinant human Caveolin-1 (rhCaveolin-1; aa1-104; Accession # Q03135).
- Format:
- Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
- Extra Details:
- Caveolin-1 is a palmitoylated 22kD membrane-associated protein in caveolae which are cholesterol-rich invaginations in the plasma membrane involved in vesicular transport and the regulation of lipid rafts and signal transduction. Caveolin-1 expression is dysregulated during cancer progression and exhibits both positive and negative effects on tumor progression. The central region of Caveolin-1 (aa105-125) is buried in the lipid layer, while the N- and C-terminal flanking regions are exposed to the cytoplasm and interact with many other proteins. Alternate splicing in human, mouse, and rat Caveolin-1 generates an isoform with a deletion of the N-terminal 31 residues. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice