C2097-77D
Cathepsin S (Cathepsin-S, CATS, CTSS, FLJ50259, MGC3886) (Not for Export EU)
Size
£698.00
- SKU:
- C2097-77D
- Application:
- IHC-Fr
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- Synthetic peptide corresponding to aa298-331 of human Cathepsin S.
- Format:
- Supplied as a lyophilized powder. Reconstitute with 50ul sterile ddH2O.
- Extra Details:
- Applications: Suitable for use in Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Formalin-fixed paraffin): 1:500 Optimal dilutions to be determined by the researcher. Immunohistochemistry Protocol: Tissue Sample: Mouse and rat visceral fat tissue Fixative: 10% Formalin Embedding: Paraffin Negative control: Pre-immuno serum Pretreatment: Intact Blocking: 2% Normal Goat Serum Primary Antibody: C2097-77D Optimal Dilution: 1:500 (1 hour at RT) Secondary Antibody: IgG (Biotin) GtxRb (1:400, 30 min) Amplification: ABC (Vector) (1:400, 30 min) Detection system: HRP Substrate: DAB, 3 minutes Counterstained: Hematoxylin, 30 seconds Amino Acid Sequence: Asn-Ser-Trp-Gly-His-Asn-Phe-Gly-Glu-Glu-Gly-Tyr-Ile-Arg-Met-Ala-Arg-Asn-Lys-Gly-Asn-His-Cys-Gly-Ile-Ala-Ser-Phe-Pro-Ser-Tyr-Pro-Glu-Ile Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice