C2095

Catalase, Suspension, Bovine

Size

£338.00

SKU:: C2095
Molecular Weight:: 250
Purity:: ≥20,000 units/mg protein activity
Storage Conditions:: 4°C Do not freeze
Supplier:: United States Biological
Format:: Supplied as a crystalline aqueous suspension. Contains thymol as a preservative. Store in glass only.
Extra Details:: Catalase is a hemoprotein containing four subunits which decomposes peroxide. Catalytic activity is present in nearly all animal cells and organs and in aerobic microorganisms. Activity: ≥20,000 units/mg protein Unit Definition: 1 unit decomposes 1umole of hydrogen peroxide/minute at 25°C, pH 7.0. Composition: 4 subunits of equal size (MW 60,000-65,000) Quality Control: SDS-PAGE Handling: Contains thymol as a preservative. To remove thymol, measure desired amount of mixed suspension, centrifuge to collect enzyme crystals, remove supernatant. Resuspend crystals in one-half initial volume of water, respin. Discard wash supernatant and dissolve crystals in buffer of choice. Storage and Stability: May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Protect from moisture.