C2088-56J

Caspase 11 (Caspase-4, CASP-4, Caspase-11, CASP-11, Protease ICH-3, Casp4, Casp11, Caspl, Ich3)

Size

£320.00

SKU:: C2088-56J
Application:: WB
Purity:: Purified by affinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: His tagged bacterial fusion protein corresponding to p30 of caspase 11.
Clone:: Cas11.17D9
Format:: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Extra Details:: Murine caspase-11 is an upstream protease of caspase-1 and interacts with the proform of caspase-1 which in turn activates IL-1 beta. Caspase-11 is not constitutively expressed in most cell types. Its expression can be upregulated by LPS, IFN-gamma, and other pro-inflammatory stimuli. NF-kB and STAT1 were reported to be involved in the activation. Caspase-11 knock -out mice were resistant to endotoxin shock and do not produce IL-1 after stimulation with LPS. Their production of IFN-gamma was also deficient. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-2ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.