C2088-56G
Caspase 11 (Casp4, Caspase 4, Aoptosis-related cysteine peptidase, Casp11, ASP-4, Caspase-4, Caspl, Ich3, ich-3, Protease ICH-3) (Not for Export EU)
Size
£704.00
- SKU:
- C2088-56G
- Purity:
- Serum
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rabbit
- Immunogen:
- 13aa peptide (286-298aa) of Mouse Caspase-11 ~C-terminal
- Format:
- Supplied as a lyophilized powder from PBS, 0.05% sodium azide.
- Extra Details:
- Caspase-11, (also known as CASP-11, ICH-3, ICE-B) promotes IL-b processing by procaspase-1 (ICE) and therefore may also have a role in inflammatory response as evident by a 30 fold increase in CASP-11 activity by LPS. The mouse and rat CASP-11 (373aa) are most homologous to human CASP-4 (377aa) protein (ICErel-II, TX, ICH-2) and could possibly be the ortholog of human CASP-4. The pro-or zymogen form of CASP-11 is activated by Granzyme-B or cathepsin-B and the processed enzyme consists of two large p20 (20kD) and two small ~p10 (10kDa subunits. CASP-11 is highly expressed in lung and spleen, weaker in heart and very little in liver, kidney, testis and skeletal muscle but has not been detected in the brain. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blotting (1-10ug/ml for affinity pure antibody using ECL technique). ELISA: Control peptide can be used to coat ELISA plates at 1ug/ml and detected with antibodies (0.5-1ug/ml for affinity pure). Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice