Caspase 9 (ICE-LAP6, Mch6, Apaf 3)

Size

£655.00

SKU:: C2088-14
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 20aa near the center of human Caspase-9. Epitope: aa290-340. Species Sequence Homology: rat (50%)
Format:: Supplied as a liquid in PBS, 0.02% sodium azide.
Extra Details:: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFα, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues. Applications: Suitable for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunoprecipitation and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml Immunocytochemistry: 2-5ug/ml Immunofluorescence: 5-20ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice