Caspase 12 (NT)

Size

£655.00

SKU:: C2087-73
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rabbit
Immunogen:: Synthetic peptide corresponding to 16aa from mouse Caspase 12 at N-terminal.
Format:: Supplied as a liquid in PBS, 0.02% sodium azide
Extra Details:: Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved (1,2). Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is colocalized to the ER with several proteins that are involved in Alzheimer’s disease including g-secretase/presenilin and b-amyloid precursor protein (APP). Caspase-12 mediates ER stress-induced apoptosis as well as cytotoxicity induced by amyloid-b. Caspase-12 is ubiquitously expressed in mouse tissues (1). Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 10ug/ml. Requires antigen retrieval using heat treatment prior to staining. Sodium citrate buffer, pH 6.0, is recommended. Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Positive Control: Murine spleen tissue lysate, human heart/spleen tissue Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Shipping Conditions:: Blue Ice