C2087-48

Caspase 8, Cleavage Site (Asp384) (FLICE, MASH, Mch5)

Size

£691.00

SKU:: C2087-48
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Synthetic peptide corresponding to the N-terminal sequence of p10 of human caspase-8.
Clone:: 3A52(11G10)
Format:: Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.
Extra Details:: Apoptosis induced by CD95 (Fas/APO-1) and tumor necrosis factor receptor 1(TNFR1) activates caspase-8 (MACH/FLICE/Mch5), leading to the release of the active subunits p18 and p10 of caspase-8 (1-3). Activated caspase-8 is able to cleave and activate downstream caspases such as caspase-1, caspase-3, caspase-6 and caspase-7. Caspase-3 ultimately elicits the morphological hallmarks of apoptosis, including DNA fragmentation and cell shrinkage. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted primary antibody in 5% nonfat dry milk, 1X TBS, 0.1% Tween20 at 4°C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.